Florida has become the latest US state to ban transgender girls and women from participating in female sports at public schools and colleges.
The state’s Republican governor Ron DeSantis signed the measure into law on Tuesday.
The law says women and girls must play on the teams of the biological s.e.x on their birth certificate.
LGBT activists have denounced the move as “discriminatory”, with one group vowing to launch a legal challenge.
We believe that is very important that the integrity of these competitions are preserved,” Mr DeSantis said as he signed the law at a Christian school in Jacksonville city.
“We’re gonna go based on biology, not based on ideology when we’re doing sports.”
The move comes as Republican-led states push back against pro-LGBT policies supported by the administration of Democratic US President Joe Biden.
Florida’s law defines an athlete’s s.e.x as that stated on official documents at birth.
Called the Fairness in Women’s Sports Act, the law says sports teams for girls and women “may not be open to students of the male sex”.
It’s not clear whether all female athletes must now show their birth certificates to be in sports teams.
The law would not bar female athletes from playing on boys or men’s teams.
The law also allows students to take legal action against the school if they feel it has violated the act and “deprived” them “of an athletic opportunity”.
Supporters of the bill say transgender female athletes have an unfair advantage, having been designated male at birth but having since transitioned.
But critics say the banning of transgender girls and women from sports is unnecessary and discriminatory.
The law will apply to public secondary and high schools, colleges and universities when it comes into effect on 1 July.
Florida state representative Carlos Smith, a Democrat who identifies as Latino and gay, spoke out against the bill, calling it “appalling”.
“This fuels transphobia and puts vulnerable kids at risk for no good reason,” he wrote on Twitter.
Very proud of all of the trans leaders and allies who came together on short notice to speak out against and condemn @GovRonDeSantis for signing the trans sports ban into law.
When trans kids are under attack, what does Orlando do? 🏳️⚧️
Stand up! Fight back!
— Rep. Carlos G Smith (@CarlosGSmith) June 2, 2021
Another Democratic lawmaker, Shevrin Jonesit, criticised what he called the “insulting” timing of the bill’s signing on 1 June, the first day of Pride Month, an annual celebration of the LGBT community.
But Kelli Stargel, a Republican state senator who supported the bill, denied the law was discriminatory.
“This bill is very simply about making sure that women can safely compete, have opportunities and are physically be able to excel in a sport that they trained for, prepared for and work for,” Ms Stargel said.
The Human Rights Campaign (HRC), a group which defends LGBT rights, said it would challenge the measure in court.
“Gov DeSantis and Florida lawmakers are legislating based on a false, discriminatory premise that puts the safety and well-being of transgender children on the line,” the president of the group, Alphonso David, said.
“Transgender kids are kids; transgender girls are girls. Like all children, they deserve the opportunity to play sports with their friends and be a part of a team.”
We’re suing to protect trans kids in Florida. pic.twitter.com/rVE1fbFae2
— Human Rights Campaign (@HRC) June 2, 2021
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